How do you clean a thermocycler?
Clean the housing or external part of the thermal cycler with a soft cleaning cloth or paper, this should be lightly moistened with a soap solution of neutral pH, otherwise you can use the cleaning cloths of computers which can be purchased in any dedicated house ( do not use waxes or abrasives).
How does a thermal cycler work?
How does a thermal cycler work? A thermal cycler has a thermal block with holes where tubes holding the reaction mixtures are placed. The temperature of the cycler then rises and lowers in discrete, pre-programmed steps to facilitate reactions.
What is ramp rate?
The increase or reduction in output per minute in spinning mode is called the ramp rate and is usually expressed either as% per minute or MW per minute. Alternatively, ramp rates are sometimes expressed as MW / minute.
How do you set up a thermocycler?
A standard polymerase chain reaction (PCR) setup consists of four steps:
- Add required reagents or mastermix and template to PCR tubes.
- Mix and centrifuge.
- Amplify per thermo cycler and primer parameters.
- Evaluate amplified DNA by agarose gel electrophoresis followed by ethidium bromide staining.
What are the three steps in each PCR cycle?
PCR is based on three simple steps required for any DNA synthesis reaction: (1) denaturation of the template into single strands; (2) annealing of primers to each original strand for new strand synthesis; and (3) extension of the new DNA strands from the primers.
Is the T100 thermal cycler suitable for PCR?
The T100 thermal cycler’s intuitive touch screen makes running PCR easier than ever before. The performance, features, and ease of use are efficiently streamlined into a compact footprint that fits in any laboratory. With the T100 thermal cycler you can:
Where can I find the Bio-Rad instruction manual?
Bio-Rad Laboratories provides many resources for scientists. The following web sites contain useful information for running PCR and real-time PCR experiments: † Life Science Research web site (discover.bio-rad.com) This site includes links to technical notes, manuals, product information, and technical support.
What are the safe use conditions for Bio Rad?
Usage Aspect Conditions for Safe Use Rated input power 100–240 Vac, 50–60 Hz, 700 W Max Overvoltage category II Replacement fuses 10 A, 250 V, 5 x 20 mm, fast blow (qty 2) Environment Indoor use only Temperature 15–31°C Relative humidity Up to 80% (noncondensing) Altitude Up to 2,000 meters above sea level Pollution degree 2 Table 4.
How to apply for the Bio-Rad Education discount?
For more than 15 years, Bio-Rad has made science education a major priority. To support this effort, the company has implemented a discount policy that allows high school and college teaching laboratories to purchase kits, instruments, reagents, and other equipment at preferred prices. How do I apply for the education discount?
