What is maize ubiquitin promoter?
The maize ubiquitin 1 (Ubi-1) promoter has been widely used to drive constitutive transgene expression in monocotyledonous plants. However, lack of data on its activity in individual transformed wheat lines constitutes a gap in the understanding and predictability of this promoter’s performance.
What is the function of the gene’s promoter?
Promoter sequences define the direction of transcription and indicate which DNA strand will be transcribed; this strand is known as the sense strand. Many eukaryotic genes have a conserved promoter sequence called the TATA box, located 25 to 35 base pairs upstream of the transcription start site.
What is promoter in transgene?
A promoter is a critical sequence required for regulating the spatial and temporal expression pattern of a transgene. Promoter sequences are isolated from upstream regions of endogenous mammalian genes. A promoter sequence normally includes a transcriptional start site as well as transcription regulatory sequences.
Does a human gene contain a promoter?
Promoter. A promoter is a sequence of DNA needed to turn a gene on or off. The process of transcription is initiated at the promoter. Usually found near the beginning of a gene, the promoter has a binding site for the enzyme used to make a messenger RNA (mRNA) molecule.
What does the 35S promoter gene do?
The promoter of the 35S RNA is a very strong constitutive promoter responsible for the transcription of the whole CaMV genome. It is well known for its use in plant transformation. It causes high levels of gene expression in dicot plants.
What is UBC promoter?
The promoter of the polyubiquitin C gene (UBC) contains putative heat shock elements (HSEs) which are thought to mediate UBC induction upon stress.
What is the most common promoter?
Promoters determine the site of transcription initiation and direct binding of RNA polymerase II. Three types of promoter sequences have been identified in eukaryotic DNA. The TATA box, the most common, is prevalent in rapidly transcribed genes.
Which is the promoter for ubiquitin in maize?
The Zea mays c.v. B73 Ubiquitin-1 ( Z. mays c.v. B73 Ubi-1) promoter drives high levels of constitutive transgene expression in plants. Repeated use of the same Z. mays c.v. B73 Ubi-1 promoter in multi-gene constructs may also lead to gene silencing, thereby making transgenic products less efficacious.
Which is a better promoter for pubi-Cat in maize?
Expression from the Ubi-1 promoter of pUBI-CAT yields more than a 10-fold higher level of CAT activity in maize protoplasts than expression from the widely used cauliflower mosaic virus 35S promoter of a 35S-CAT construct.
What are the structure of polyubiquitin genes in maize?
Maize polyubiquitin genes: structure, thermal perturbation of expression and transcript splicing, and promoter activity following transfer to protoplasts by electroporation Plant Mol Biol. 1992 Feb;18(4):675-89.doi: 10.1007/BF00020010.
