What is HPTLC method?
High-performance thin-layer chromatography (HPTLC) is an enhanced form of thin-layer chromatography (TLC). A number of enhancements can be made to the basic method of thin-layer chromatography to automate the different steps, to increase the resolution achieved, and to allow more accurate quantitative measurements.
What is chromatographic fingerprinting?
Chromatographic fingerprinting is used for both establishing the identity and quality of the single herbs being added into herbal formulation . Nowadays chromatographic fingerprinting is used at two stages during the manufacture of a herbal formulation.
What is chromatographic fingerprinting Slideshare?
◍ A chromatographic fingerprint is a chromatographic pattern of characteristic chemical constituents present in the specific extract. ◍ Using chromatographic techniques , a profile of various chemical constituents is obtained .
What is the difference between HPLC and HPTLC?
The main difference between HPLC and HPTLC is that HPLC is the enhanced form of liquid chromatography, whereas HPTLC is the enhanced form of thin-layer chromatography. Moreover, HPLC consists of a closed system, while HPTLC is an open system.
What is a marker compound?
Marker compound means chemical constituents within a medicinal that can be used to verify its potency or identity. For sometimes, the marker compounds may be described as active ingredients or chemicals that confirm the correct botanical identity of the starting material.
Why is HPTLC used?
HPTLC is used for purity control of chemicals, pesticides, steroids, and water analysis. [50] HPTLC is also widely used for analysis of vitamins, water-soluble food dyes, pesticides in fruits, vegetables, and other food stuffs.
How is chromatography used in fingerprinting?
It’s used to separate long molecules, like proteins, RNA and DNA from one another. Different organisms have different DNA sequences, so electrophoresis can be used to identify organisms and for DNA fingerprinting. Chromatography is also used to separate different molecules, usually pigments.
Which method is used for determination of heavy metal in herbal materials?
The most commonly used methods to determine heavy metals in herbal materials include atomic absorption spectrometry (AAS), inductively coupled plasma-optical emission spectrometry (ICP-OES) or inductively coupled plasma-mass spectrometry (ICP-MS), and X-ray fluorescence spectrometry (XRFS) [76] .
What are the applications of HPTLC?
What does a C18 column mean?
C18 columns are HPLC (high performance liquid chromatography) columns that use a C18 substance as the stationary phase. C18 simply means that the molecules contain 18 carbon atoms, so the other atoms in the molecule can vary, leading to significantly different substances.
