What is cytospin centrifugation?
A cytospin is obtained by employing centrifugal force to isolate, concentrate and deposit a monolayer of cells from a dilute cell suspension onto a circular area on a slide. The objective is to keep cells intact enabling the morphology of the cells to be examined.
Which mode of staining is used in slide preparation?
Two types of stain methods were used in this study: Giemsa and methylene blue/eosin. For methylene blue/eosin staining, the slides were fixed by placing 2 drops of fixing solutions on the slides and air dried in a biological hood (2 min) or on bench (3–5 min).
What is the meaning of cytospin?
A cytocentrifuge, sometimes referred to as a cytospin, is a specialized centrifuge used to concentrate cells in fluid specimens onto a microscope slide so that they can be stained and examined.
What happens during centrifugation?
A centrifuge works by using the principle of sedimentation: Under the influence of gravitational force (g-force), substances separate according to their density. Different types of separation are known, including isopycnic, ultrafiltration, density gradient, phase separation, and pelleting.
How many slides can be centrifuged in a Cytospin?
Place the mounted holder in the corresponding recess in the Cytospin. A maximum of twelve slides can be centrifuged at once. Remember that the Cytospin must always be balanced. Prepare a cell suspension of not more than 0.5 x 10 6 cells/ml in the protein-containing medium.
How is a Cytospin obtained in a cell?
A cytospin is obtained by employing centrifugal force to isolate, concentrate and deposit a monolayer of cells from a dilute cell suspension onto a circular area on a slide. The objective is to keep cells intact enabling the morphology of the cells to be examined.
How many drops of cytospin in a cytofunnel?
We recommend putting two and three drops (corresponding to 100–200µl of cell suspension, respectively) in different cytofunnels to establish the optimal volume necessary to result in a good monolayer of cells with no overlapping of cells or cell clumps. The loading volume should be optimized for each cell line used.
How does cytocentrifugation of body fluid samples work?
Cytocentrifugation (also known as “cytospin”) provides the best method for both concentrating cells in body fluid samples and maintaining cellular morphology. The cytospin process works by wicking fluid into a filter while fluid samples are spun into a central column and deposited in a mono-layer onto a defined area of a slide.
