What is Gblock?
gBlocks™ Gene Fragments are double-stranded DNA fragments of 125–3000 bp in length. They are the industry standard for double-stranded gene fragments, designed for affordable and easy gene construction or modification, applications such as antibody research and CRISPR-mediated genome editing, qPCR standards, and more.
How do you use Gblock?
Resuspending gBlocks Gene Fragments
- Before opening the tube, spin it down in a microcentrifuge for 3–5 seconds to ensure the DNA is in the bottom of the tube.
- Add molecular grade water, or a buffer such as IDTE, to reach a final concentration of 10 ng/µL.
- Vortex briefly.
- Incubate at approximately 50°C for 15–20 min.
What is Gblock gene fragments?
Custom DNA fragments can jump-start your research gBlocks Gene Fragments are sequence verified DNA fragments that can be used in any application requiring double-stranded DNA. They can greatly accelerate your research. Once ordered, the target fragments are available in as few as 2 business days.
What is Ultramer oligo?
Ultramer DNA Oligos are long, single- and double-stranded synthetic DNA sequences. They are manufactured with an optimized synthesis platform that achieves consistent coupling efficiencies over 99.5%. In vitro transcription—templates for the synthesis of RNA.
How do you synthesize a gene?
Unlike DNA replication that occurs in cells or by Polymerase Chain Reaction (PCR), gene synthesis does not require a template strand. Rather, gene synthesis involves the step-wise addition of nucleotides to a single-stranded molecule, which then serves as a template for creation of a complementary strand.
How does codon optimization work?
What is Codon Optimization? Codons represent the genetic code that transfers information from genes to mRNA to protein. Codon optimization is a process used to improve gene expression and increase the translational efficiency of a gene of interest by accommodating codon bias of the host organism.
What is a Megamer?
Megamer Single-Stranded DNA Fragments are sequence-verified, single-stranded DNA strands of length 201–2000 bases. They can be used in applications such as homology-directed repair of CRISPR-mediated genome editing, in vitro transcription, and more.
Can scientists create DNA?
A living organism has been made using entirely human-made DNA. It is an important step in synthetic biology with scientists creating a synthetic genome four times larger and more complex than any genomes they have previously engineered.
Why do we use codon optimization?
How do you optimize codons?
Codon-optimization describes gene engineering approaches that use synonymous codon changes to increase protein production. Applications for codon-optimization include recombinant protein drugs and nucleic acid therapies, including gene therapy, mRNA therapy, and DNA/RNA vaccines.
How are gene fragments made in GBlocks DNA oligos?
They are manufactured with the same industry-leading, high-fidelity synthesis chemistries that were developed for our Ultramer™ DNA Oligos. Each gBlocks Gene Fragment goes through a quality control process, which includes size verification by capillary electrophoresis and sequence identification by mass spectrometry.
How big are Gblocks double stranded DNA fragments?
Pure confidence gBlocks™ Gene Fragments are double-stranded DNA fragments of 125–3000 bp in length. They are the industry standard for double-stranded gene fragments, designed for affordable and easy gene construction or modification, applications such as antibody research and CRISPR-mediated genome editing, qPCR standards, and more.
Can you order GBlocks gene fragments without 5 ′ phosphate group?
For added flexibility, you can order gBlocks Gene Fragments with or without a 5′-phosphate group.
How big are the Gblocks HiFi gene fragments?
gBlocks HiFi Gene Fragments are double-stranded DNA fragments with sizes between 1000–3000 bp and verified with a median error rate of less than 1:12,000 via NGS.
