What is Tol2?
Abstract. The medaka fish Tol2 element is an autonomous transposon that encodes a fully functional transposase. The transposase protein can catalyze transposition of a transposon construct that has 200 and 150 base pairs of DNA from the left and right ends of the Tol2 sequence, respectively.
Are zebrafish transgenic?
Zebrafish have been increasingly used for transgenesis studies mainly due to easy egg accessibility and manipulation together with relatively short generation time. The zebrafish transgenic technology becomes very useful when coupled to continuous in vivo observation of the vertebrate embryonic vasculature.
Why do we use zebrafish in research?
One reason that zebrafish are an important biomedical model is because zebrafish embryos are transparent and they develop outside of the uterus. This unique developmental process allows scientists to study the details of development starting from fertilization and continuing throughout development.
Why is the Tol2 transposon system so useful?
Due to the high efficiency and abilities to carry larger foreign DNA fragments, Tol2 transposon system is discovered to be useful in not only the establishment of stable expression cell strain but also the generation of transgenic mice and rats.
How does the tol2kit use site specific recombination?
The Tol2kit uses site-specific recombination-based cloning ( multisite Gateway technology) to allow quick, modular assembly of promoter-coding sequence-3′ tag constructs in a Tol2 transposon backbone.
Where does the Tol2 transposase plasmid come from?
Since the destination vectors and transposase plasmid are all derived from Dr. Koichi Kawakami’s original constructs, we ask that you please first contact Dr. Kawakami and execute an MTA for use of the Tol2 transposase construct and plasmids based on Tol2.
How big can a Tol2 transposon be cloned?
Tol2 transposon shows no preferences with respect to the integration sites which means theoretically it can translocate to anywhere on a chromosome. Single copy insertions can be achieved and DNA inserts of fairly large sizes (as large as 13 kilobases) can be cloned between these sequences.
