What does SV40 antigen do?
SV40 large T antigen (Simian Vacuolating Virus 40 TAg) is a hexamer protein that is a dominant-acting oncoprotein derived from the polyomavirus SV40. TAg is a product of an early gene transcribed during viral infection by SV40, and is involved in viral genome replication and regulation of host cell cycle.
What is the role of large t antigen in SV40 pathogenesis?
Large T antigen also functions to initiate and maintain viral DNA replication and to activate transcription from the late promoter. As the level of the structural proteins rises, progeny virions are assembled. In culture, cell death occurs about 96 h postinfection.
What is viral T antigen?
The large and small tumor antigens (T antigens) are the major regulatory proteins encoded by SV40. Large T antigen is responsible for both viral and cellular transcriptional regulation, virion assembly, viral DNA replication, and alteration of the cell cycle.
What is SV40 promoter?
The early promoter of the simian virus 40 (SV40) has been used as a model eukaryotic promoter for the study of DNA sequence elements and cellular factors that are involved in transcriptional control and initiation. Some of these elements are present in cellular genes, and may exhibit tissue-specificity in their action.
What is immortalization of cell?
An immortalised cell line is a population of cells from a multicellular organism which would normally not proliferate indefinitely but, due to mutation, have evaded normal cellular senescence and instead can keep undergoing division.
Can there be 2 Ori?
Two different Ori helps the plasmid to replicate in different host organisms. Thus the same vector can be directly propagated from one host to another. Its is a type of shuttle vector.
What is the function of T region of SV40 viral vector?
What is polyomavirus infection?
Polyomaviruses are small, nonenveloped DNA viruses, which are widespread in nature. In immunocompetent hosts, the viruses remain latent after primary infection. With few exceptions, illnesses associated with these viruses occur in times of immune compromise, especially in conditions that bring about T cell deficiency.