What is agglutination inhibition?
Agglutination inhibition or hemagglutination inhibition refers to the inhibition of these reactions by soluble antigen which reacts with the combining sites of the antibodies and thereby prevents their binding to and agglutination of the particles.
What does a positive agglutination test mean?
Agglutination of red blood cells in a given site indicates a positive identification of the blood antigens: in this case, A and Rh antigens for blood type A-positive.
What is the hemagglutination inhibition test used for?
The hemagglutination inhibition (HI) assay for influenza A virus has been used since the 1940s. The assay may be utilized to detect or quantify antibodies to influenza A viruses and can be used to characterize differences in antigenic reactivity between influenza isolates.
What is the principle of haemagglutination inhibition test?
The principle behind the hemagglutination test is that the nucleic acids of viruses encode proteins, such as hemagglutinin, that are expressed on the surface of the virus (Figs.
What is meant by haem agglutination inhibition test?
The hemagglutination inhibition (HI) assay is used to titrate the antibody response to a viral infection. The HI assay takes advantage of some viruses’ ability to hemagglutinate (bind) red blood cells, therefore forming a “lattice” and preventing the red blood cells from clumping.
What is Hemadsorption inhibition test?
A quantitative hemadsorption-inhibition test was developed to estimate myxovirus serum antibodies within 24 h by determining the serum dilution inhibiting hemadsorption in 50% of the infected cells.
Which viruses can cause hemagglutination?
Hemagglutination is a reaction that causes clumping of red blood cells in presence of some enveloped viruses, such as the influenza virus. A glycoprotein on the viral surface, namely hemagglutinin, interacts with red blood cells, leading to the clumping of red blood cells and the formation of a lattice.
What is the principle of agglutination?
Principle. Agglutination is the development of antigen–antibody complexes in the form of particle clumps (agglutinates) due to the interaction between the insoluble form of antigens (i.e., antigen associated with latex particles) and its soluble and specific antibodies (Fig. 3.5) [1, 2].
