Is hemochromatosis stained by Prussian blue?
A Prussian blue iron stain demonstrates the blue granules of hemosiderin in hepatocytes and Kupffer cells. Hemochromatosis can be primary (the cause is probably an autosomal recessive genetic disease) or secondary (excess iron intake or absorption, liver disease, or numerous transfusions).
What is Prussian blue stain used for?
Iron Stain Kit (Prussian Blue stain) ab150674 is intended for use in the detection of ferric iron in tissues, blood smears, or bone marrow smears. Ferric iron is normally found in small amounts in bone marrow and the spleen. Abnormally large deposits may be seen in hemochromatosis and hemosiderosis.
What pigment does Prussian blue stain?
Hemosiderin is an iron-containing, golden-brown, granular pigment derived from ferritin, the initial iron-storage protein. As iron accumulates within the cell, aggregates of ferritin molecules form hemosiderin (E-Fig.
How do you remove Prussian blue stain?
Many methods of performing Perls Prussian blue stain for iron have been published, Drury and Wallington (1980) give a protocol that uses a mixture of 1 part 2% hydrochloric acid and 1 part 2% potassium ferrocyanide that is applied to the section for 20–30 minutes followed by a rinse in distilled water and application …
What is the Prussian blue test?
Perls Prussian blue is a commonly used method in histology, histopathology, and clinical pathology to detect the presence of iron in tissue or cell samples. The method stains mostly iron in the ferric state which includes ferritin and hemosiderin, rather than iron in the ferrous state.
What is in Prussian blue?
The pigment Prussian blue consists of iron cations, cyanide anions, and water. Thus, the formula that gives a truer idea of its composition is Fe4[Fe(CN)6]3. Its formal name is iron(III) hexacyanoferrate(II). As shown in the two left-hand drawings, the Fe(CN)6 anion in Prussian blue is octahedral.
What does Prussian blue measure?
Prussian blue is a common histopathology stain used by pathologists to detect the presence of iron in biopsy specimens, such as in bone marrow samples. Iron deposits in tissue then form the purple Prussian blue dye in place, and are visualized as blue or purple deposits.
What is Prussian blue made of?
The pigment Prussian blue consists of iron cations, cyanide anions, and water. The empirical formula—minus the water of crystallization—is Fe7(CN)18. This seems odd with respect to the iron oxidation state until you learn that the complex contains Fe(II) and Fe(III).
Why is the liver stained with Perls Prussian blue?
Section of liver stained with Perls Prussian blue, showing iron accumulations (blue) consistent with homozygous genetic hemochromatosis. Perls’s method is used to indicate “non- heme ” iron in tissues such as ferritin and hemosiderin, the procedure does not stain iron that is bound to porphyrin forming heme such as hemoglobin and myoglobin.
What kind of iron is in Perls Prussian blue?
The method stains mostly iron in the ferric state which includes ferritin and hemosiderin, rather than iron in the ferrous state. Section of liver stained with Perls Prussian blue, showing iron accumulations (blue) consistent with homozygous genetic hemochromatosis.
Why do you use Perls Prussian blue on bone marrow?
Perls Prussian blue is commonly used on bone marrow aspirates to indicate levels of iron storage and may provide reliable evidence of iron deficiency. Perls did not publish a detailed procedure other than indicating a dilute potassium ferrocyanide solution was applied to the tissue followed by hydrochloric acid.
When was the Perls Prussian blue reaction first used?
In some tissues iron is tightly bound to proteins and cannot be demonstrated . In such cases, if treated with hydrogen peroxide, iron is released and then demonstrated by using Perls Prussian blue reaction. It was discovered in 1706 and was first used as synthetic colour in paints by Diesbach in Berlin.
