Where did HL60 cells come from?
Cell line history HL60 is a human cell line derived from peripheral blood lymphocytes of a 36 year old woman suffering from acute promyleocytic leukaemia. It was one of the first human myeloid leukaemia cells to be established as a continuous suspension cell culture which had previously been difficult to achieve.
What are HL60 cells and why are they interesting in the study of apoptosis?
HL-60 cell model was used to study the effect of DNA topoisomerase (topo) IIα and IIβ on differentiation and apoptosis of cells and is especially useful in dielectrophoresis studies, which require an aqueous environment with suspended and round cells.
How do you subculture a human leukemia cell line HL60?
Subculture Routine Take a sample of the cell suspension, e.g. 100ul, to count cells. Centrifuge the cell suspension at low speed i.e. 100 – 150 x g for a maximum of 5 mintues. Remove medium and resuspend the cell pellet at a density of 3 – 5 x 100,000 cells/ml in fresh medium containing 20% serum.
Are HL60 cells adherent?
The Ad-HL60 cells have a more differentiated phenotype than the non-adherent cells from which they were derived, and synthesized 1.88 +/- 0.07 (+/- S.E.M.) pmol 24,25-(OH)2D3/h per 10(6) cells following culture in RPMI-1640 medium containing less than 0.02 nM 1 alpha,25-(OH)2D3.
Why are HL 60 cells used?
The HL-60 cell line has proven useful in the study of cell formation and differentiation of normal and of course leukemic cells. This is because HL-60 cells can be differentiated through the myeloid pathway to cells such as neutrophils quite easily using DMSO, DMF and other inducers such as retinoic acid.
Why are HL 60 cells important?
Are cells immortal?
Embryonic stem cells and germ cells have also been described as immortal. Immortal cell lines of cancer cells can be created by induction of oncogenes or loss of tumor suppressor genes.
Where did the HL-60 cell line come from?
Description: The HL-60 cell line was derived from peripheral blood leukocytes obtained by leukopheresis of a 36-year-old Caucasian female with acute promyelocytic leukemia. It was among the first long-term suspension cultures of human myeloid leukaemic cells to be established.
What kind of research is HL 60 used for?
Jump to navigation Jump to search. The HL-60 cell line is a human leukemia cell line that has been used for laboratory research on blood cell formation and physiology.
How long does it take HL 60 to double?
HL60. The HL-60 cell line is a human leukemia cell line that has been used for laboratory research on blood cell formation and physiology. HL-60 proliferates continuously in suspension culture in nutrient and antibiotic chemicals. The doubling time is about 36–48 hours.
What kind of morphology do HL 60 cells have?
HL-60 cells predominantly show neutrophilic promyelocytic morphology. Subsequent evaluation, including the karyotype that showed absence of the defining t (15;17) translocation, concluded that HL-60 cells are from a case of AML FAB-M2 (now referred to as AML with maturation (WHO)).