How do you prepare van Gieson stain?
For nervous tissues may be prepared as follows:
- Deparaffinize and hydrate slides to distilled water.
- Stain in Verhoeff’s solution for 1 hour.
- Rinse in tap water with 2-3 changes.
- Differentiate in 2% ferric chloride for 1-2 minutes.
What tissue components does Van Gieson’s stain?
Van Gieson’s stain is a mixture of picric acid and acid fuchsin. It is the simplest method of differential staining of collagen and other connective tissue. It was introduced to histology by American neuropsychiatrist and pathologist Ira Van Gieson.
What special care should be taken when using Van Gieson?
Wash with plenty of water. If skin irritation occurs: Get medical advice/attention. lungs. Get medical attention.
What stain is used for bone?
A wide array of special stains for bone such as the Von Kossa, tartrate-resistant acid phosphatase (TRAP), alkaline phosphatase, Gomori trichrome, safranin O and toluidine blue stains are performed on a routine basis.
How is Van Gieson stain used in connective tissue?
Connective Tissue. •. Elastin stain. —. Elastin van Gieson (EVG) stain highlights elastic fibers in connective tissue. —. EVG stain is useful in demonstrating pathologic changes in elastic fibers, such as reduplication, breaks or splitting that may result from episodes of vasculitis, or connective tissue disorders such as Marfan syndrome.
What kind of stain is Verhoeff Van Gieson stain?
Verhoeff stain component: an iron-haematoxylin stain that is specific for elastic fibers. It forms strong bonds with elastin, the main component of elastic connective tissue.
Why is Verhoeff Van Gieson used for histology?
Although there are numerous special stains for identification of elastic fibers, VVG is most commonly used because it is quick, and produces intense staining of elastic fibers. General principles of the stain
How is sodium thiosulfate used in Van Gieson stain?
Sodium thiosulfate is used to remove excess iodine, and the van Gieson counterstain is used to produce contrast with the haematoxylin stain. The dye is attracted to the large volume of mordant in the differentiating solution and removed from the tissue.