What is an HPLC frit?

What is an HPLC frit?

Inert physical filtration devices, typically installed before pre-columns, that provide a coarse filtration of the HPLC eluent and prevent contaminant particles from reaching the HPLC system.

What is a PTFE frit?

PTFE frits and frit material are used to retain ion exchange resin within Savillex microcolumns and in a wide variety of other sample preparation applications. PTFE allows for organic solvent filtration and is chemically inert, non-leaching, and non-reactive for high sensitivity analyses.

How do you flush Agilent HPLC?

Change seal wash solvent (10 % / 90 % isopropanol/water) and bottle. Flush all channels with water at 2.5 – 3 mL/min for 5 min to remove salt deposits if buffer applications were used.

What is a column frit?

Column frits is a porous frit, which is widely used for column protection. It keeps the packing material in the column. Choose frits with 0.5 μm pores to retain packings prepared from 3 μm particles, frits with 2 μm pores to retain larger particles.

What causes RSD failure in HPLC?

Column temperature fluctuations (especially evident in ion exchange systems). Column overloading. (Retention times usually decrease as mass of solute injected on column exceeds column capacity.) Sample solvent incompatible with mobile phase.

Why we do seal wash in HPLC?

The routine use of highly concentrated buffer solutions (100 mM) will reduce the life of seals and pistons in your pump. The use of seal wash in HPLC systems helps to increase the performance and lifetime of the piston seals.

How do you clean a column?

Wash the column with the current mobile phase without buffer, salt or acid for 10-15 min. If the ratio of organic solvent and water is changed, salt may precipitate. Wash impurities in a column to achieve a stable base line: Use a solvent with strong elution properties in which the sample dissolves well.

What is frit used for?

Frits form an important part of the batches used in compounding enamels and ceramic glazes; the purpose of this pre-fusion is to render any soluble and/or toxic components insoluble by causing them to combine with silica and other added oxides.

What does Fritting mean?

transitive verb. 1 : to prepare (materials for glass) by heat : fuse. 2 : to convert into a frit.

Which pump is used in HPLC?

reciprocating pumps
Most HPLC pumps are reciprocating pumps. The solvent is drawn into a small chamber (with the solvent check valve open) and pumped out of it (when the column check valve is open) by the back and forth motion of a motor driven piston.

What kind of pump does a Agilent 1200 HPLC use?

Agilent 6430 LC/MS with 1200 HPLC System System Includes: Agilent 6430A LC/MSESI SourceE2M28 Roughing PumpAgilent 1200 HPLCG1322/G1379 DegasserG1311/G1312 your choice of Quaternary or Binary pumpG136 Agilent 1200 HPLC with DAD & Quat Pump This Agilent 1200 HPLC with DAD & Quat Pump is in excellent condition.

What kind of liquid handler does Agilent use?

Agilent 1200 Preparative HPLC with CTC IFC PAL Liquid Handler This is a Pre-owned Agilent 1200 Preparative HPLC with CTC IFC PAL Liquid Handler. System includes: Agilent G1361A PREP PUMPS (2)Agilent

What kind of pistons are used in HPLC pumps?

Piston seals are spring loaded and engineered to deliver optimal performance over highly dynamic flow and pressure ranges Seals are manufactured from a proprietary polymer blend and feature a spring made from the same high-quality stainless steel that is used in our pump’s flow path

Which is the best HPLC system for high throughput?

Agilent 1200 HPLC. The Agilent 1200 Series binary LC gradient system is your first choice when your requirements demand the maximum in gradient and low flow rate accuracy and precision. It is the ideal system for all high-throughput and fast applications.

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