What is NP-40 lysis buffer?
NP40 Cell Lysis Buffer is a high-quality, ready-to-use lysis buffer suitable for the preparation of cell extracts for ELISA, western blotting, and antibody bead immunoassays (Luminex) applications. The formulation helps retain the protein structure and function needed for enzyme assays or immunoassays.
What is SDS lysis buffer?
SDS (sodium dodecyl sulfate) lysis buffer SDS is ionic denaturing detergent. Hot SDS buffer is often used when the proteins need to be completely solubilized and denatured.
What is IP buffer?
IP Lysis Buffer is a mammalian whole cell lysis reagent based on a modified RIPA buffer formulation without SDS. This moderate-strength lysis buffer effectively solubilizes cellular proteins but does not liberate genomic DNA or disrupt protein complexes like ordinary RIPA buffer.
What is the function of NP-40 detergent?
NP-40 is a nonionic polyoxyethylene surfactant that is most frequently used as a component of cell lysis buffers or other solutions intended to extract and solubilize proteins. Surfact-Amps Detergent Solutions (10% w/v) provide unsurpassed purity, quality and stability.
How many antibodies do you need for CoIP?
You want to start with a fair amount of material; aim for between 1 and 3 mg of total protein for every 0.2-0.5 ml of your starting sample volume. You should also aim to keep your target protein as happy as possible throughout the disruptive procedure of cell or tissue lysis.
Which is the best lysis buffer for np40?
Quantity 100 mL Description NP40 Cell Lysis Buffer is suitable for the preparation of cell extracts to be analyzed by Antibody Bead Immunoassay, ELISA, and western blotting. Buffer Formulation 50 mM Tris, pH 7.4 250 mM NaCl 5 mM EDTA 50 mM NaF 1 mM Na3VO4.
What makes up the Pierce IP lysis buffer?
Pierce IP Lysis Buffer is composed of 25 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1% NP-40 and 5% glycerol. The buffer does not contain protease or phosphatase inhibitors; however, if desired, inhibitors, such as Thermo Scientific Halt Protease Inhibitor Cocktail or Phosphatase Inhibitor Cocktail can be added just before use…
Is the Thermo Scientific lysis buffer similar to RIPA buffer?
Is the Thermo Scientific IP Lysis Buffer similar to RIPA buffer? The Thermo Scientific IP Lysis Buffer (Cat. No. 87787) is a mammalian whole cell lysis buffer based on a modified RIPA buffer formulation without SDS.
When to add phosphatase to Thermo Scientific lysis buffer?
We recommend inhibitors, such as Thermo Scientific Halt Protease Inhibitor Cocktail (Cat. No. 87786) or Phosphatase Inhibitor Cocktail (Cat. No. 78420) to be added to the buffer just before use. Is the Thermo Scientific IP Lysis Buffer similar to RIPA buffer?
