What media does Legionella grow on?
Legionella is best grown on BCYE agar containing 0.1% α-ketoglutarate and L-cysteine supplemented with dyes to aid identification of colonies and antimicrobial agents such as vancomycin, polymyxin B, and anisomycin to inhibit overgrowth by other bacteria and yeast; incubation is at 35°C in a humidified atmosphere …
Which media is used to culture L. pneumophila?
The first protocol describes the use of buffered charcoal yeast extract (BCYE) agar, the solid medium that is most commonly used for culturing L. pneumophila.
Does Legionella grow on chocolate agar?
Numerous reports in the literature have described Legionella isolation on selective supplemented media such glycine-vancomycin-polymyxin B-cycloheximide agar or BCYE supplemented with glycine, vancomycin, polymyxin B, and anisomycin, but only a few have described Legionella isolation on routinely used enriched media …
How do you isolate Legionella?
The standard method of detection of Legionella spp. in environmental sample is culture on Buffered Charcoal Yeast Extract (BCYE) agar supplemented with L-cysteine [11, 12].
What color is Legionella?
Legionella micro-colonies should appear green colour and Legionella pneumophila micro-colonies appear red. Twenty-one environmental water samples analysed by standard culture plus five control samples (Two sterile water samples with Legionella as positive controls and three sterile water samples as negative controls).
What grows on BCYE Agar?
Buffered charcoal yeast extract (BCYE) agar is a selective growth medium used to culture or grow certain types of bacteria, particularly the Gram-negative species Legionella pneumophila. It has also been used for the laboratory diagnosis of Acanthamoeba keratitis, Francisella and Nocardia.
How is Legionella treated in water?
Other methods to control Legionella include copper and silver ionisation and biocide treatments (eg chlorine dioxide). To ensure that they remain effective their application will need suitable assessment as part of the overall water treatment programme including proper installation, maintenance and monitoring.
What kind of broth is used to grow Legionella?
Legionella are grown either in supplemented buffered yeast extract (BYE) broth or in supplemented buffered charcoal-yeast extract agar (BCYE). Antimicrobial agents are added in increasing concentrations. MICs and MBCs are assessed at 1-5 days of incubation.
What kind of broth to use for l.pneumophila?
The next procedure details the cultivation of L. pneumophila in buffered yeast extract (BYE) broth, i.e., the liquid medium version of BCYE agar. We describe how culturing in BYE broth can also be used for investigating proteins that are secreted by the type II secretion system of L. pneumophila.
What kind of Media do you use for Legionella?
The first protocol describes the use of buffered charcoal yeast extract (BCYE) agar, the solid medium that is most commonly used for culturing L. pneumophila. The next procedure details the cultiva … This chapter describes methods for culturing Legionella pneumophila in both complex and defined media.
How are antimicrobial agents used to test for Legionella?
Susceptibility of Legionella species to antimicrobial drugs has been based on three laboratory methods: standard dilution testing in agar or broth, intracellular models in vitro, and animal models of Legionella infection.
