How do you inoculate bacteria culture?
Using a sterile pipette tip or toothpick, select a single colony from your LB agar plate. Drop the tip or toothpick into the liquid LB + antibiotic and swirl. Loosely cover the culture with sterile aluminum foil or a cap that is not air tight. Incubate bacterial culture at 37°C for 12-18 hr in a shaking incubator.
What is inoculation in culture media?
Inoculation is the study of introducing microorganisms into environments where they will grow and reproduce. In other words, we can say that inoculation means introducing a certain substance into another substance. For example, inoculation is adding a certain type of nutrient or chemical into a suspension of bacteria.
What is an inoculation method?
Inoculation, process of producing immunity and method of vaccination that consists of introduction of the infectious agent onto an abraded or absorptive skin surface instead of inserting the substance in the tissues by means of a hollow needle, as in injection.
How do you inoculate agar plates with bacteria?
To inoculate the agar, lift the lid of the Petri dish and tilt. Do not fully remove or place on the desk as the lid prevents micro-organisms from the air contaminating the culture, and vice versa. Following inoculation, the lid of the Petri dish should be secured in place by strips of adhesive tape for safety reasons.
What are the types of inoculation?
Different types of inoculation media are agar plates, broth culture, slant culture, plate culture, stab culture, etc., which has been discussed in detail below.
What is an example of inoculation?
For example, an inoculation message designed to discourage teen cigarette smoking (e.g., Pfau et al., 1992) might begin with a warning that peer pressure will strongly challenge their negative attitudes toward smoking, then follow this forewarning with a handful of potential counterarguments they might face from their …
What is inoculation and what is its purpose?
1 : the act or process or an instance of inoculating especially : the introduction of a pathogen or antigen into a living organism to stimulate the production of antibodies.
