How do you scale-up a bioreactor?

How do you scale-up a bioreactor?

The traditional method for scale-up of a fermentation process involves determining the reactor geometry, impeller speed, and aeration rate of the large-scale bioreactor on the basis of the experimental results of the lab-scale bioreactor.

What is cell culture scale-up?

The objectives of cell culture scale-up are to maximize cell growth and productivity by optimizing the process parameters. Process optimization requires consideration of the cell type, nutrient medium, feeds, and bioreactor settings while minimizing the production of byproducts.

What is scale-up and scale down in bioreactor?

… Scale-down bioreactors have been applied in bioprocess development to study the response of microbial cell factories and other expression systems to various stress-inducing concentration gradients (scale-up effects) that occur in large-scale bioreactors (Neubauer & Junne, 2016; Nienow et al., 2013).

What are the different methods to scale-up animal cell culture?

Scale-up in suspension is the preferred method as it is simpler. Scale-up of suspension culture primarily involves an increase in the volume of the culture. Small scale generally means the culture capacity less than 2 litres volume (or sometimes 5 litres).

What is scale up in bioprocessing?

These bioprocesses are usually developed at small laboratory scale. Later, the established processes are stepwise transferred to larger volumes until the final industrial production-scale is reached. This procedure is known as scale-up.

What is scale up and scale?

What is Scale up vs Scale out? Scaling out, or horizontal scaling, contrasts to scaling out, or vertical scaling. Scaling up, in contrast, is making a component larger or faster to handle a greater load. This would be moving your application to a virtual server (VM) with 2 CPU to one with 3 CPUs.

How do cell bioreactors work?

During the bioprocess, the bioreactor feeds a sterile gas mixture such as air into the culture medium. Constant stirring not only distributes the nutrients – it also reduces the size of the gas bubbles that arise in the culture vessel, thus efficiently releasing oxygen into the nutrient solution.

What is meant by cell culture?

Listen to pronunciation. (sel KUL-cher) The growth of microorganisms such as bacteria and yeast, or human, plant, or animal cells in the laboratory. Cell cultures may be used to diagnose infections, to test new drugs, and in research.

What do you mean by scale down?

scaled down. DEFINITIONS1. to make something smaller in size, amount etc than it used to be. The search operation has been scaled down.

What is scale down process?

The goal when scaling down is to create a small-scale or lab-scale system that mimics the performance of its large-scale (pilot or manufacturing) counterpart, when both the process parameters are varied within their operating ranges and also when a process parameter deviates outside its operating range.

How do you scale-up production?

How to Successfully Scale Your Manufacturing Business

  1. Know Your Financial Condition.
  2. Invest in Production.
  3. Be Realistic with Your Sales Forecast.
  4. Hire the Right People.
  5. Maintain a Network of Existing Clients or Suppliers.

How is scale-up achieved in bioprocess technology?

Can a cell culture be scaled up to a bioreactor?

Cell culture of Sf9 was scaled up to a 1000 L bioreactor directly by this strategy. Animal cell cultivations are widely used for producing antibodies, vaccines, and recombinant protein drugs. However, the extreme sensitivity of animal cells to hydrodynamic stress often hinders its scale-up in large-scale stirred tank bioreactors.

When do you need a scale-up analysis for CHO cell culture?

Scale-up analysis for a CHO cell culture process in large-scale bioreactors Bioprocess scale-up is a fundamental component of process development in the biotechnology industry. When scaling up a mammalian cell culture process, it is important to consider factors such as mixing time, oxygen transfer, and carbon dioxide removal.

How does a single use bioreactor scale up?

They can also reduce water use and validation requirements. In the poster, authors used BioBLU Single-Use Vessels of different sizes (maximum working volumes of 0.25 L, 3.75 L, and 40 L) that are of geometrically similar stirred-tank design to scale up a mAb CHO process from small to pilot scale production.

When to scale up a mammalian cell culture process?

When scaling up a mammalian cell culture process, it is important to consider factors such as mixing time, oxygen transfer, and carbon dioxide removal. In this study, cell-free mixing studies were pe …

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